Journal: Science advances

Article Title: Cationic nanoparticles enhance T cell tumor infiltration and antitumor immune responses to a melanoma vaccine.

doi: 10.1126/sciadv.abk3150

Figure Lengend Snippet: Fig. 5. In vitro stimulation of BMDCs. BMDCs were incubated for 2 days with the indicated treatment (as described in Materials and Methods), and then cells and supernatants were harvested for analysis of surface marker expression and cytokine secretion, respectively. (A) Graph showing relative CD40 expression. (B) Graph showing relative CD80 expression. (C) Graph showing percent CD86hi expression. (D) Graph showing relative MHC class I expression. (E) Graph showing IL-6 secretion levels. (F) Graph showing difference in IP-10 secretion. Data are plotted as means ± SD. Probability values are determined by one-way ANOVA with Tukey posttest.

Article Snippet: BMDCs were then incubated with one or more of the following antibodies: anti-mouse CD11c fluorescein isothiocyanate (FITC) (clone N418, Invitrogen), anti-mouse CD40 APC (clone 3/23, BioLegend, San Diego, CA), anti-mouse CD80 APC (16-10A1), antimouse CD86 APC (20-0862, Tonbo Biosciences, San Diego, CA), and anti-mouse MHC class I (H-2Kb) PE (AF6-88.5.5.3, Invitrogen, Waltham, MA), for 30 min on ice in the dark (72).

Techniques: In Vitro, Incubation, Marker, Expressing